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Anti-EIF4E TrueMAB Antibody clone 6C11
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for EIF4E (NM_001968)
|Full length human recombinant protein of human EIF4E (NP_001959) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB 1:500~2000, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1|
|CBP; EIF4E1; EIF4EL1; EIF4F|
|All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.
Flow cytometric Analysis of Hela cells, using anti-EIF4E antibody(TA502993),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-EIF4E antibody(TA502993),(Red), compared to a nonspecific negative control antibody,(Blue).