Anti-SOCS3 Antibody, clone 3D3

Also for SOCS3 (NM_003955)

OriGene Data

Immunogen	Full length human recombinant protein of human SOCS3 (NP_003946) produced in HEK293T cell.
Clone Name	clone 3D3	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens suppressor of cytokine signaling 3 (SOCS3)
Alternative Name	ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3
Database Link	NP_003946
Function	This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq].
Related Pathway	Adipogenesis EGFR1 Signaling Pathway Jak-STAT signaling pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOCS3 mouse monoclonal antibody. (TA502991)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOCS3 mouse monoclonal antibody. (TA502991)

IF Image

Anti-SOCS3 mouse monoclonal antibody (TA502991) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305 ).

FC Image

Flow cytometric Analysis of Hela cells, using anti-SOCS3 antibody(TA502991),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-SOCS3 antibody(TA502991),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-SOCS3 TrueMAB Antibody clone 3D3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data