OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PDF antibodies

Anti-PDF TRUEMAB Antibody clone 3F3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502988
  • PDF mouse monoclonal antibody, clone 3F3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC411712) , 20ug Explanation
100ul 379 In Stock
CF502988
  • Carrier-free (BSA/glycerol-free) PDF mouse monoclonal antibody, clone 3F3
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
Add to Shopping Cart
WB(2)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human PDF (NP_071736) produced in E.coli.
Clone Nameclone 3F3 IsotypeIgG2b
Species ReactivityHuman Concentration0.78 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 19.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens peptide deformylase (mitochondrial) (PDF)
Alternative Name
Database LinkNP_071736
Entrez Gene 64146 Human
FunctionProtein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
WB Image
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PDF monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
IF Image
Anti-PDF mouse monoclonal antibody (TA502988) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ).
FC Image
HEK293T cells transfected with either RC205788 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDF antibody(TA502988), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-PDF antibody(TA502988),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PDF antibody(TA502988),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title