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Anti-LIMK1 TRUEMAB Antibody Clone OTI8C8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502978 LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI8C8 (formerly 8C8) 100ul 325
In Stock
LC400838 LIMK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502978 Carrier-free (BSA/glycerol-free) LIMK1 mouse monoclonal antibody, clone OTI8C8 (formerly 8C8) 100ug $450 3-4 weeks
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Also for LIMK1 (NM_002314)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
Clone NameClone OTI8C8 IsotypeIgG2a
Species ReactivityHuman Concentration0.76 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 72.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
Alternative NameLIMK; LIMK-1
Database LinkNP_002305
Entrez Gene 3984 Human
FunctionThere are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Related PathwayProtein KinaseDruggable Genome Axon guidanceFc gamma R-mediated phagocytosisRegulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502978)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502978)
Anti-LIMK1 mouse monoclonal antibody (TA502978) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA502978), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-LIMK1 antibody(TA502978) (Red), compared to a nonspecific negative control antibody (Blue).


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