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Home Antibody All anti-SNAP25 antibodies

Anti-SNAP25 TRUEMAB Antibody clone 1G2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502967
  • SNAP25 mouse monoclonal antibody, clone 1G2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418912) , 20ug
100ul 325 In Stock
CF502967
  • Carrier-free (BSA/glycerol-free) SNAP25 mouse monoclonal antibody, clone 1G2
100ug $450 3-4 weeks
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WB(1)
IHC(1)
IF(1)
FC(2)
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Also for SNAP25 (NM_003081)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.
Clone Nameclone 1G2 IsotypeIgG1
Species ReactivityHuman Concentration0.68 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1
Alternative NamebA416N4.2; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25
Database LinkNP_003072
FunctionSynaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008].
Related Pathway

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (RC202068, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502967)
IF Image
Anti-SNAP25 mouse monoclonal antibody (TA502967) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25(RC202068).
FC Image
HEK293T cells transfected with either RC202068 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SNAP25 antibody(TA502967), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-SNAP25 antibody(TA502967),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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