LGR5 Mouse Monoclonal Antibody [Clone ID: OTI2H7]

CAT#: TA502948

LGR5 mouse monoclonal antibody, clone OTI2H7 (formerly 2H7)

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502948)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502948)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502948)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502948)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5. Positive lysates LY401213 (100ug) and LC401213 (20ug) can be purchased separately from OriGene.

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  Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA502948, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502948)

• Flow cytometric analysis of living 293T cells transfected with LGR5 overexpression plasmid (RC212825), Red) using anti-LGR5 antibody (TA502948). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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Specifications

Product Data

• Clone Name: OTI2H7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in 293T Cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.72 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 99.8 kDa
• Gene Name: leucine rich repeat containing G protein-coupled receptor 5
• Database Link:
  NP_003658
  Entrez Gene 14160 MouseEntrez Gene 299802 RatEntrez Gene 8549 Human
  UniProt ID O75473
• Synonyms: FEX; GPR49; GPR67; GRP49; HG38

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane