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Home Antibody All anti-AP2M1 antibodies

Anti-AP2M1 TRUEMAB Antibody Clone OTI1C12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502917 AP2M1 mouse monoclonal antibody, clone OTI1C12 (formerly 1C12) 100ul $325 In Stock
LC422484 AP2M1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502917 Carrier-free (BSA/glycerol-free) AP2M1 mouse monoclonal antibody, clone OTI1C12 (formerly 1C12) 100ug $450 3-4 weeks
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WB(2)
FC(1)
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Also for AP2M1 (NM_001025205)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human AP2M1 (NP_001020376) produced in HEK293T cell.
Clone NameClone OTI1C12 IsotypeIgG2a
Species ReactivityHuman Concentration0.63 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:2000, FLOW 1:100
Predicted MW Explanation 49.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2
Alternative NameAP50; CLAPM1; mu2
Database LinkNP_001020376
Entrez Gene 1173 Human
FunctionThis gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayDruggable Genome EndocytosisHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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HEK293T cells transfected with either RC201377 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AP2M1 antibody(TA502917), and then analyzed by flow cytometry.
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