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Anti-RPS6KB1 TRUEMAB Antibody Clone OTI1G4
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for RPS6KB1 (NM_003161)
|Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (RC217324, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502913)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502913)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502913)
Anti-RPS6KB1 mouse monoclonal antibody (TA502913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1(RC217324).
HEK293T cells transfected with either RC217324 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPS6KB1 antibody(TA502913), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RPS6KB1 antibody(TA502913),(Red), compared to a nonspecific negative control antibody,(Blue).