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Home Antibody All anti-LIMK1 antibodies

Anti-LIMK1 TRUEMAB Antibody Clone 3H6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502904
  • LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, Clone 3H6
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400838) , 20ug
100ul 325 In Stock
CF502904
  • Carrier-free (BSA/glycerol-free) LIMK1 mouse monoclonal antibody,clone 3H6
100ug $450 3-4 weeks
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WB(2)
IHC(2)
IF(1)
FC(1)
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Also for LIMK1 (NM_002314)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
Clone NameClone 3H6 IsotypeIgG2b
Species ReactivityHuman , Rat , Monkey , Mouse Concentration1.00 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
Alternative NameLIMK; LIMK-1
Database LinkNP_002305
FunctionThere are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Related Pathway

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIMK1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502904)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502904)
IF Image
Anti-LIMK1 mouse monoclonal antibody (TA502904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
FC Image
HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA502904), and then analyzed by flow cytometry.

 

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