Anti-ETF1 TrueMAB Antibody clone 2E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

SKU	Description	Amount	Price	Availability*
TA502891	ETF1 (eRF1) mouse monoclonal antibody, clone 2E3 FREE positive control: HEK293T cell transient overexpression lysate (LC401492) , 20ug	100ul	$379	In Stock
CF502891	Carrier-free (BSA/glycerol-free) ETF1 mouse monoclonal antibody, clone 2E3	100ug	$450	3-4 weeks
TA802519S	Purified GAPDH mouse monoclonal antibody (Loading control), clone 2D9	30ul	$100 $50*	In Stock

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

Also for ETF1 (NM_004730)

OriGene Data

Immunogen	Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
Clone Name	clone 2E3	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, FC	Suggested Dilutions	WB 1:500, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens eukaryotic translation termination factor 1 (ETF1), transcript variant 1
Alternative Name	D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1
Database Link	NP_004721
Function	This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012].
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (RC220387 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.

FC Image

Flow cytometric Analysis of Hela cells, using anti-ETF1 antibody(TA502891),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-ETF1 antibody(TA502891),(Red), compared to a nonspecific negative control antibody,(Blue).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

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