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Anti-ADH1B TRUEMAB Antibody Clone OTI5D7
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for ADH1B (NM_000668)
|Full length human recombinant protein of human ADH1B (NP_000659) produced in HEK293T cell.|
|Human , Monkey
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), transcript variant 1|
Entrez Gene 125 Human
Entrez Gene 707908 Monkey
|The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008].|
|Druggable Genome Glycolysis / GluconeogenesisFatty acid metabolismTyrosine metabolismRetinol metabolismMetabolism of xenobiotics by cytochrome P450Drug metabolism - cytochrome P450More Pathways >> |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADH1B (RC205391 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADH1B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ADH1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502778)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502778)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502778)
Anti-ADH1B mouse monoclonal antibody (TA502778) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADH1B(RC205391 ).
HEK293T cells transfected with either RC205391 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ADH1B antibody(TA502778), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ADH1B antibody(TA502778),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ADH1B antibody(TA502778),(Red), compared to a nonspecific negative control antibody,(Blue).