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Anti-STAT5A TRUEMAB Antibody Clone OTI8H1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for STAT5A (NM_003152)
|Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens signal transducer and activator of transcription 5A (STAT5A), transcript variant 2|
Entrez Gene 6776 Human
|The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].|
|Stem cell relevant signaling - DSL/Notch pathwayStem cell relevant signaling - JAK/STAT signaling pathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome ErbB signaling pathwayJak-STAT signaling pathwayPathways in cancerChronic myeloid leukemiaAcute myeloid leukemia|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (RC205753, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.
Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-STAT5A monoclonal antibody.(1:200)
Anti-STAT5A mouse monoclonal antibody (TA502737) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT5A(RC205753).
HEK293T cells transfected with either RC205753 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-STAT5A antibody(TA502737), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-STAT5A antibody(TA502737),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-STAT5A antibody(TA502737),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).