Anti-PDHA1 Antibody, clone 2B10

Also for PDHA1 (NM_000284)

OriGene Data

Immunogen	Full length human recombinant protein of human PDHA1 (NP_000275) produced in HEK293T cell.
Clone Name	clone 2B10	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1
Alternative Name	PDHA; PDHCE1A; PHE1A
Database Link	NP_000275
Function	The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (RC201831, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.

IF Image

Anti-PDHA1 mouse monoclonal antibody (TA502704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831).

FC Image

HEK293T cells transfected with either RC201831 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDHA1 antibody(TA502704), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-PDHA1 antibody(TA502704),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-PDHA1 TrueMAB Antibody clone 2B10

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OriGene Data

Reference Data