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Anti-ARFGAP1 TRUEMAB Antibody Clone OTI 2C10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502635 ARFGAP1 mouse monoclonal antibody, clone OTI 2C10 (formerly 2C10) 100ul 325
In Stock
LC406240 ARFGAP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502635 Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone OTI 2C10 (formerly 2C10) 100ug $450 3-4 weeks
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Also for ARFGAP1 (NM_175609)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
Clone NameClone OTI 2C10 IsotypeIgG2b
Species ReactivityHuman Concentration0.4 mg/ml
Guaranteed Application *WB, IF, FC Suggested Dilutions WB 1:2000, IF 1:100, FLOW 1:100,
Predicted MW Explanation 45.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2
Alternative NameARF1GAP; HRIHFB2281
Database LinkNP_783202
Entrez Gene 55738 Human
FunctionThe protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul
Related Pathway Endocytosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IF Image
Anti-ARFGAP1 mouse monoclonal antibody (TA502635) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1(RC206987).

FC Image
HEK293T cells transfected with either RC206987 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARFGAP1 antibody(TA502635), and then analyzed by flow cytometry.


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