HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502546), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-NECAB1 antibody(TA502546),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-NECAB1 antibody(TA502546),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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