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Home Antibody All anti-NNMT antibodies

Anti-NNMT TRUEMAB Antibody Clone OTI2A3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502538 NNMT mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) 100ul $325 In Stock
LC401860 NNMT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502538 Carrier-free (BSA/glycerol-free) NNMT mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) 100ug $450 3-4 weeks
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WB(2)
IHC(1)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human NNMT (NP_006160) produced in HEK293T cell.
Clone NameClone OTI2A3 IsotypeIgG2b
Species ReactivityHuman Concentration0.16 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 29.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nicotinamide N-methyltransferase (NNMT)
Alternative Namenicotinamide N-methyltransferase
Database LinkNP_006160
Entrez Gene 4837 Human
FunctionN-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq].
Related Pathway Nicotinate and nicotinamide metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502538)
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Anti-NNMT mouse monoclonal antibody (TA502538) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT(RC200641).
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HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502538), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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