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Anti-NNMT TRUEMAB Antibody Clone OTI2A3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NNMT (NM_006169)
|Full length human recombinant protein of human NNMT (NP_006160) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502538)
Anti-NNMT mouse monoclonal antibody (TA502538) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT(RC200641).
HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502538), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody(TA502538),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).