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Anti-GBA3 TRUEMAB Antibody Clone OTI5G5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502533 GBA3 mouse monoclonal antibody, clone OTI5G5 (formerly 5G5) 100ul 325
In Stock
LC402815 GBA3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502533 Carrier-free (BSA/glycerol-free) GBA3 mouse monoclonal antibody, clone OTI5G5 (formerly 5G5) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3(NP_066024) produced in E.coli.
Clone NameClone OTI5G5 IsotypeIgG1
Species ReactivityHuman Concentration0.9 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:200 - 1:1000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 53.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glucosidase, beta, acid 3 (gene/pseudogene) (GBA3), transcript variant 1
Alternative NameCBG; CBGL1; GLUC; KLRP
Database LinkNP_066024
Entrez Gene 57733 Human
FunctionGBA3, or cytosolic beta-glucosidase (EC, is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM].
Related Pathway Cyanoamino acid metabolismStarch and sucrose metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3.

WB Image
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GBA3 monoclonal antibody.(1:200)

IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502533)

FC Image
HEK293T cells transfected with either RC211035 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBA3 antibody(TA502533), and then analyzed by flow cytometry.

FC Image
Flow cytometric Analysis of Jurkat cells, using anti-GBA3 antibody(TA502533),(Red), compared to a nonspecific negative control antibody,(Blue).


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