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Anti-GBA3 TRUEMAB Antibody Clone OTI5G5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for GBA3 (NM_020973)
|Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3(NP_066024) produced in E.coli.|
|WB, IHC, FC
||WB: 1:200 - 1:1000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens glucosylceramidase beta 3 (gene/pseudogene) (GBA3), transcript variant 1, coding|
|CBG; CBGL1; GLUC; KLRP|
Entrez Gene 57733 Human
|GBA3, or cytosolic beta-glucosidase (EC 18.104.22.168), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 22.214.171.124), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM].|
| Cyanoamino acid metabolismStarch and sucrose metabolism|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GBA3 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502533)
HEK293T cells transfected with either RC211035 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBA3 antibody(TA502533), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-GBA3 antibody(TA502533),(Red), compared to a nonspecific negative control antibody,(Blue).