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Anti-RASSF1 TrueMAB Antibody Clone 9F5
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for RASSF1 (NM_007182)
|Human recombinant protein fragment corresponding to amino acids 1-341 of human RASSF1(NP_009113) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens Ras association (RalGDS/AF-6) domain family member 1 (RASSF1), transcript variant A|
|123F2; NORE2A; RASSF1A; RDA32; REH3P21|
|This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (RC213525, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.
Anti-RASSF1 mouse monoclonal antibody (TA502510) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525).
HEK293T cells transfected with either RC213525 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RASSF1 antibody(TA502510), and then analyzed by flow cytometry.