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Home Antibody All anti-UBA2 antibodies

Anti-UBA2 TRUEMAB Antibody Clone OTI4A3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502504 UBA2 mouse monoclonal antibody, clone OTI4A3 (formerly 4A3) 100ul
30ul
$379 In Stock
LC417267 UBA2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502504 Carrier-free (BSA/glycerol-free) UBA2 mouse monoclonal antibody, clone OTI4A3 (formerly 4A3) 100ug $450 3-4 weeks
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WB(3)
IHC(4)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human UBA2 (NP_005490) produced in HEK293T cell.
Clone NameClone OTI4A3 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.41 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 71 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin-like modifier activating enzyme 2 (UBA2)
Alternative NameARX; HRIHFB2115; SAE2
Database LinkNP_005490
Entrez Gene 10054 Human
Entrez Gene 308508 Rat
Entrez Gene 705808 Monkey
Entrez Gene 476490 Dog
FunctionPosttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM].
Related Pathway Ubiquitin mediated proteolysis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA2 (RC201288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBA2 monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-UBA2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-UBA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502504)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UBA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502504)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502504)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-UBA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502504)
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Anti-UBA2 mouse monoclonal antibody (TA502504) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBA2(RC201288).
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