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Anti-POLR2E TRUEMAB Antibody clone 3C5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for POLR2E (NM_002695)
|Full length human recombinant protein of human POLR2E (NP_002686) produced in HEK293T cell.|
||WB 1:500~2000, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)|
|hRPB25; hsRPB5; RPABC1; RPB5; XAP4|
|This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
Anti-POLR2E mouse monoclonal antibody (TA502488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266).