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Home Antibody All anti-POLR2E antibodies

Anti-POLR2E TRUEMAB Antibody clone 3C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502488
  • POLR2E mouse monoclonal antibody, clone 3C5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419161) , 20ug Explanation
100ul 325 In Stock
CF502488
  • Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody, clone 3C5
100ug $450 3-4 weeks
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WB(2)
IF(1)
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Also for POLR2E (NM_002695)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POLR2E (NP_002686) produced in HEK293T cell.
Clone Nameclone 3C5 IsotypeIgG2a
Species ReactivityHuman Concentration0.74 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:500~2000, IF 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)
Alternative NamehRPB25; hsRPB5; RPABC1; RPB5; XAP4
Database LinkNP_002686
FunctionThis gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
IF Image
Anti-POLR2E mouse monoclonal antibody (TA502488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266).

 

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