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Anti-BTRC TRUEMAB Antibody Clone 2H2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BTRC (NM_033637)
|Human recombinant protein fragment corresponding to amino acids 52-354 of human BTRC(NP_378663) produced in E.coli.|
|WB, IF, FC
|| WB 1:1000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC), transcript variant 1|
|BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1|
Entrez Gene 8945 Human
|This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar |
|Druggable Genome Oocyte meiosisUbiquitin mediated proteolysisWnt signaling pathwayHedgehog signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTRC (RC207025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BTRC monoclonal antibody at 1:200 dilution.
Anti-BTRC mouse monoclonal antibody (TA502465) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTRC(RC207025).
HEK293T cells transfected with either RC207025 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BTRC antibody(TA502465), and then analyzed by flow cytometry.