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Home Antibody All anti-ACOT12 antibodies

Anti-ACOT12 TRUEMAB Antibody Clone OTI1B9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502462 ACOT12 mouse monoclonal antibody, clone OTI1B9 (formerly 1B9) 100ul $379 In Stock
LC408930 ACOT12 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502462 Carrier-free (BSA/glycerol-free) ACOT12 mouse monoclonal antibody, clone OTI1B9 (formerly 1B9) 100ug $450 3-4 weeks
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WB(1)
IHC(7)
FC(2)
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Also for ACOT12 (NM_130767)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ACOT12 (NP_570123) produced in HEK293T cell.
Clone NameClone OTI1B9 IsotypeIgG2a
Species ReactivityHuman, Monkey Concentration0.91 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100
Predicted MW Explanation 61.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens acyl-CoA thioesterase 12 (ACOT12)
Alternative NameCach; CACH-1; STARD15; THEAL
Database LinkNP_570123
Entrez Gene 134526 Human
Entrez Gene 711665 Monkey
Function
Related Pathway Pyruvate metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502462)
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HEK293T cells transfected with either RC210445 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACOT12 antibody(TA502462), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody(TA502462),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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