Home Antibody All anti-POLR2J2 antibodies
Anti-POLR2J2 TRUEMAB Antibody Clone OTI3G4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for POLR2J2 (NM_032959)
|Full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2)|
Entrez Gene 246721 Human
|This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq]. |
|Transcription Factors Purine metabolismPyrimidine metabolismMetabolic pathwaysRNA polymeraseHuntington's disease|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR2J2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502454)
Anti-POLR2J2 mouse monoclonal antibody (TA502454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2(RC224755).
HEK293T cells transfected with either RC224755 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2J2 antibody(TA502454), and then analyzed by flow cytometry.