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Home Antibody All anti-PRKCE antibodies

Anti-PRKCE TRUEMAB Antibody clone 1B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502417
  • PRKCE (PKC epsilon ) mouse monoclonal antibody, clone 1B4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401658) , 20ug Explanation
100ul
30ul
379 In Stock
CF502417
  • Carrier-free (BSA/glycerol-free) PRKCE mouse monoclonal antibody, clone 1B4
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(3)
IHC(4)
IF(1)
FC(3)
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Also for PRKCE (NM_005400)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
Clone Nameclone 1B4 IsotypeIgG2b
Species ReactivityHuman , Dog , Monkey Concentration0.88 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 83.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein kinase C, epsilon (PRKCE)
Alternative NamenPKC-epsilon; PKCE
Database LinkNP_005391
Entrez Gene 5581 Human
Entrez Gene 714533 Monkey
Entrez Gene 609934 Dog
FunctionProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome Vascular smooth muscle contractionTight junctionFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosisType II diabetes mellitus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (RC217702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PRKCE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PRKCE monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502417)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502417)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502417)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502417)
IF Image
Anti-PRKCE mouse monoclonal antibody (TA502417) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE(RC217702).
FC Image
HEK293T cells transfected with either RC217702 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKCE antibody(TA502417), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-PRKCE antibody(TA502417),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PRKCE antibody(TA502417),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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