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Anti-GPHN TrueMAB Antibody clone 7C6
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for GPHN (NM_020806)
|Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell. |
|Human , Monkey
||0.5~1.0 mg/ml (Lot Dependent)
|| WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens gephyrin (GPHN), transcript variant 1|
|GEPH; GPH; GPHRYN; HKPX1|
|This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502283), and then analyzed by flow cytometry.