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Home Antibody All anti-ATP6V1F antibodies

Anti-ATP6V1F TRUEMAB Antibody clone 1B8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502279
  • ATP6V1F mouse monoclonal antibody, clone 1B8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418132) , 20ug
100ul 325 In Stock
CF502279
  • Carrier-free (BSA/glycerol-free) ATP6V1F mouse monoclonal antibody, clone 1B8
100ug $450 3-4 weeks
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WB(1)
IHC(9)
IF(1)
FC(1)
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Also for ATP6V1F (NM_004231)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
Clone Nameclone 1B8 IsotypeIgG2a
Species ReactivityHuman Concentration1.00 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F), transcript variant 1
Alternative NameATP6S14; VATF; Vma7
Database LinkNP_004222
FunctionThis gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502279)
IF Image
Anti-ATP6V1F mouse monoclonal antibody (TA502279) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1F(RC210728).
FC Image
HEK293T cells transfected with either RC210728 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATP6V1F antibody(TA502279), and then analyzed by flow cytometry.

 

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