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OriGene Antibodies in recent publications
A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [anti-DDK]

Excitotoxicity Upregulates SARM1 Protein Expression and Promotes Wallerian-Like Degeneration of Retinal Ganglion Cells and Their Axons Invest. Ophthalmol. Vis. Sci., Apr 2013; 54: 2771 - 2780. [SARM1]

Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX Nature 497, 122-126 doi:10.1038/nature12052 [anti-DDK]

Natriuretic Peptide Receptor-3 Gene (NPR3): Nonsynonymous Polymorphism Results in Significant Reduction in Protein Expression Because of Accelerated Degradation Circ Cardiovasc Genet, Apr 2013; 6: 201 - 210. [NPR3]

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Home All anti-ATP6V1F antibodies

Anti-ATP6V1F TrueMAB Antibody clone 1B8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA502279
  • Purified ATP6V1F mouse monoclonal antibody, clone 1B8
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418132) , 20ug
100ul $325 In Stock Add to Shopping Cart
CF502279
  • Carrier-free (BSA/glycerol-free) ATP6V1F mouse monoclonal antibody, clone 1B8
100ug $450 3-4 weeks Add to Shopping Cart
WB(1)
IHC(9)
IF(1)
FC(1)
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Also for ATP6V1F (NM_004231)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F), transcript variant 1
Synonyms:ATP6S14; VATF; Vma7
ImmunogenFull length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 1B8 IsotypeIgG2a
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
BackgroundThis gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATP6V1F mouse monoclonal antibody. (TA502279)
IF Image
Anti-ATP6V1F mouse monoclonal antibody (TA502279) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1F(RC210728).
FC Image
HEK293T cells transfected with either RC210728 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATP6V1F antibody(TA502279), and then analyzed by flow cytometry.

 

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