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Home Antibody All anti-DNAJB1 antibodies
KO-Validated

Anti-DNAJB1 TRUEMAB Antibody Clone OTI1G4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502244 DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1G4 (formerly 1G4) 100ul $379 In Stock
LC401851 DNAJB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502244 Carrier-free (BSA/glycerol-free) DNAJB1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4) 100ug $450 4 Weeks
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WB(3)
IF(1)
FC(2)
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Also for DNAJB1 (NM_006145)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
Clone NameClone OTI1G4 IsotypeIgG2a
Species ReactivityHuman , Dog , Monkey Concentration0.97 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 37.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameDnaJ heat shock protein family (Hsp40) member B1
Alternative NameHSPF1|Hdj1|Hsp40|RSPH16B|Sis1
Database LinkNP_006136
Entrez Gene 3337 Human
Entrez Gene 718890 Monkey
Entrez Gene 476688 Dog
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody.
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and DNAJB1-Knockout 293T cells (KO, Cat# LC811758) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1 monoclonal antibody TA502244,(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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IF Image
Anti-DNAJB1 mouse monoclonal antibody (TA502244) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762).
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FC Image
HEK293T cells transfected with either RC201762 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJB1 antibody(TA502244), and then analyzed by flow cytometry.
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FC Image
Flow cytometric Analysis of Hela cells, using anti-DNAJB1 antibody(TA502244),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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