Anti-MSI1 Antibody, clone 1G8

Also for MSI1 (NM_002442)

Gene Name	Homo sapiens musashi RNA-binding protein 1 (MSI1)
Synonyms:	musashi 1; musashi homolog 1 (Drosophila)
Immunogen	Full length human recombinant protein of human MSI1 (NP_002433) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1G8	Isotype	IgG2a
Species Reactivity	Human , Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Background	This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI1 (RC215992, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSI1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MSI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MSI1 mouse monoclonal antibody. (TA502198)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MSI1 mouse monoclonal antibody. (TA502198)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MSI1 mouse monoclonal antibody. (TA502198)

IF Image

Anti-MSI1 mouse monoclonal antibody (TA502198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MSI1(RC215992).

FC Image

HEK293T cells transfected with either RC215992 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MSI1 antibody(TA502198), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-MSI1 antibody(TA502198),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-MSI1 TrueMAB Antibody clone 1G8

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