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Home Antibody All anti-NUMB antibodies

Anti-NUMB TRUEMAB Antibody Clone OTI4F3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502168 NUMB mouse monoclonal antibody, clone OTI4F3 (formerly 4F3) 100ul $379 In Stock
LC401229 NUMB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502168 Carrier-free (BSA/glycerol-free) NUMB mouse monoclonal antibody, clone OTI4F3 (formerly 4F3) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(16)

OriGene Data

ImmunogenFull length human recombinant protein of human NUMB(NP_003735) produced in HEK293T cell.
Clone NameClone OTI4F3 IsotypeIgG2a
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 69.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens numb homolog (Drosophila) (NUMB), transcript variant 3
Alternative NameC14orf41; c14_5527; S171
Database LinkNP_003735
Entrez Gene 8650 Human
FunctionThe protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway Notch signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NUMB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168)
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