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Anti-MAPK7 TrueMAB Antibody clone 2E2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for MAPK7 (NM_002749)
|Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens mitogen-activated protein kinase 7 (MAPK7), transcript variant 3|
|BMK1; ERK4; ERK5; PRKM7|
|The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (RC203506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.
Anti-MAPK7 mouse monoclonal antibody (TA502148) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506).
HEK293T cells transfected with either RC203506 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAPK7 antibody(TA502148), and then analyzed by flow cytometry.