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Anti-LMAN1 TRUEMAB Antibody clone 1A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502137
  • LMAN1 mouse monoclonal antibody, clone 1A8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401709) , 20ug
100ul 379 In Stock
CF502137
  • Carrier-free (BSA/glycerol-free) LMAN1 mouse monoclonal antibody, clone 1A8
100ug $450 3-4 weeks
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WB(3)
IHC(6)
IF(1)
FC(2)
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Also for LMAN1 (NM_005570)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
Clone Nameclone 1A8 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.84 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens lectin, mannose-binding, 1 (LMAN1)
Alternative NameERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60
Database LinkNP_005561
FunctionThe protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-LMAN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502137)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502137)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502137)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502137)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502137)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502137)
IF Image
Anti-LMAN1 mouse monoclonal antibody (TA502137) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1(RC207088).
FC Image
HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LMAN1 antibody(TA502137), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-LMAN1 antibody(TA502137),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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