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Home Antibody All anti-NHEJ1 antibodies

Anti-NHEJ1 TRUEMAB Antibody Clone OTI3E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502005 NHEJ1 (XLF) mouse monoclonal antibody, clone OTI3E5 (formerly 3E5) 100ul $325 In Stock
LC403031 NHEJ1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502005 Carrier-free (BSA/glycerol-free) NHEJ1 mouse monoclonal antibody, clone OTI3E5 (formerly 3E5) 100ug $450 3-4 weeks
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WB(2)
FC(1)
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Also for NHEJ1 (NM_024782)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
Clone NameClone OTI3E5 IsotypeIgG1
Species ReactivityHuman Concentration0.59 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:2000, Flow: 1:100
Predicted MW Explanation 33.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nonhomologous end-joining factor 1 (NHEJ1)
Alternative NameXLF
Database LinkNP_079058
Entrez Gene 79840 Human
FunctionDouble-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq].
Related Pathway Non-homologous end-joining

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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HEK293T cells transfected with either RC203393 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NHEJ1 antibody(TA502005), and then analyzed by flow cytometry.
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