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Home Antibody All anti-SSSCA1 antibodies

Anti-SSSCA1 TRUEMAB Antibody clone 2F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501915
  • SSSCA1 mouse monoclonal antibody, clone 2F5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416665) , 20ug Explanation
100ul 379 In Stock
CF501915
  • Carrier-free (BSA/glycerol-free) SSSCA1 mouse monoclonal antibody, clone 2F5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IF(1)
FC(3)
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Also for SSSCA1 (NM_006396)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.
Clone Nameclone 2F5 IsotypeIgG1
Species ReactivityHuman Concentration0.48 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 21.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Sjogren syndrome/scleroderma autoantigen 1 (SSSCA1)
Alternative Namep27
Database LinkNP_006387
Entrez Gene 10534 Human
FunctionThis antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSSCA1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) at 1:200 dilution.
IF Image
Anti-SSSCA1 mouse monoclonal antibody (TA501915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1(RC201946).
FC Image
HEK293T cells transfected with either RC201946 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSSCA1 antibody(TA501915), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-SSSCA1 antibody(TA501915),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-SSSCA1 antibody(TA501915),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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