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Anti-SSSCA1 TRUEMAB Antibody clone 2F5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SSSCA1 (NM_006396)
|Full length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens Sjogren syndrome/scleroderma autoantigen 1 (SSSCA1)|
Entrez Gene 10534 Human
|This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSSCA1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) at 1:200 dilution.
Anti-SSSCA1 mouse monoclonal antibody (TA501915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1(RC201946).
HEK293T cells transfected with either RC201946 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSSCA1 antibody(TA501915), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SSSCA1 antibody(TA501915),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SSSCA1 antibody(TA501915),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).