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Anti-ZIM2 TRUEMAB Antibody clone 5E12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501906
  • ZIM2 mouse monoclonal antibody, clone 5E12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402427) , 20ug Explanation
100ul $325 In Stock
CF501906
  • Carrier-free (BSA/glycerol-free) ZIM2 mouse monoclonal antibody, clone 5E12
100ug $450 3-4 weeks
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WB(1)
IHC(6)
IF(1)
FC(1)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-150 and 428-527 of human ZIM2 (NP_056178) produced in E.coli.
Clone Nameclone 5E12 IsotypeIgG1
Species ReactivityHuman Concentration0.37 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, Flow: 1:100
Predicted MW Explanation 61.0 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens zinc finger, imprinted 2 (ZIM2), transcript variant 1
Alternative NameZNF656
Database LinkNP_056178
Entrez Gene 23619 Human
FunctionIn human, ZIM2 and PEG3 (GeneID:5178) are two distinct genes that share a set of 5' exons and have a common promoter, and both genes are paternally expressed. Alternative splicing events connect the shared exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. This is in contrast to mouse and cow, where ZIM2 and PEG3 genes do not share exons in common, and the imprinting status of ZIM2 is also not conserved amongst mammals. Additional 5' alternatively spliced transcripts encoding the same protein have been found for the human ZIM2 gene. [provided by RefSeq].
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZIM2 (RC211188, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZIM2.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZIM2 mouse monoclonal antibody. (TA501906)
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ZIM2 mouse monoclonal antibody. (TA501906)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZIM2 mouse monoclonal antibody. (TA501906)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ZIM2 mouse monoclonal antibody. (TA501906)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ZIM2 mouse monoclonal antibody. (TA501906)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ZIM2 mouse monoclonal antibody. (TA501906)
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Anti-ZIM2 mouse monoclonal antibody (TA501906) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ZIM2(RC211188).
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HEK293T cells transfected with either RC211188 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ZIM2 antibody(TA501906), and then analyzed by flow cytometry.
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