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Anti-RNH1 TRUEMAB Antibody clone 3F11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for RNH1 (NM_002939)
|Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
||WB 1:500~2000, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1|
|Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNH1 (RC208360, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNH1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNH1 monoclonal antibody.
HEK293T cells transfected with either RC208360 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNH1 antibody(TA501875), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RNH1 antibody(TA501875),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).