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Home Antibody All anti-MICAL1 antibodies

Anti-MICAL1 TRUEMAB Antibody Clone OTI2H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501846 MICAL1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) 100ul
30ul
$379 In Stock
LC402940 MICAL1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501846 Carrier-free (BSA/glycerol-free) MICAL1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) 100ug $450 3-4 weeks
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WB(2)
IHC(7)
IF(1)
FC(2)
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Also for MICAL1 (NM_022765)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MICAL1 (NP_073602) produced in HEK293T cell.
Clone NameClone OTI2H1 IsotypeIgG2b
Species ReactivityHuman Concentration0.93 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 117.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1
Alternative NameMICAL; MICAL-1; NICAL
Database LinkNP_073602
Entrez Gene 64780 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
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Anti-MICAL1 mouse monoclonal antibody (TA501846) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
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HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501846), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody(TA501846),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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