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Home Antibody All anti-BEST3 antibodies

Anti-BEST3 TRUEMAB Antibody Clone OTI1B6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501826 anti-BEST3 (Bestrophin 3) mouse monoclonal antibody, clone OTI1B6 (formerly 1B6) 100ul $325 In Stock
LC407540 BEST3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501826 Carrier-free (BSA/glycerol-free) BEST3 mouse monoclonal antibody, clone OTI1B6 (formerly 1B6) 100ug $450 3-4 weeks
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WB(1)
IF(1)
FC(1)
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Also for BEST3 (NM_152439)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell.
Clone NameClone OTI1B6 IsotypeIgG2b
Species ReactivityHuman Concentration0.76 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 50.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens bestrophin 3 (BEST3), transcript variant 2
Alternative NameVMD2L3
Database LinkNP_689652
Entrez Gene 144453 Human
FunctionBEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM].
Related PathwayIon Channels: OtherTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BEST3 (RC218436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BEST3.
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Anti-BEST3 mouse monoclonal antibody (TA501826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436).
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HEK293T cells transfected with either RC218436 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BEST3 antibody(TA501826), and then analyzed by flow cytometry.
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