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Anti-ARHGAP25 TRUEMAB Antibody Clone OTI4A9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501748 anti-ARHGAP25 mouse monoclonal antibody, clone OTI4A9 (formerly 4A9) 100ul 325
In Stock
LC402385 ARHGAP25 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501748 Carrier-free (BSA/glycerol-free) ARHGAP25 mouse monoclonal antibody, clone OTI4A9 (formerly 4A9) 100ug $450 3-4 weeks
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Also for ARHGAP25 (NM_014882)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.
Clone NameClone OTI4A9 IsotypeIgG1
Species ReactivityHuman Concentration0.23 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100
Predicted MW Explanation 72.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2
Alternative NameHEL-S-308; KAIA0053
Database LinkNP_055697
Entrez Gene 9938 Human
FunctionARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (RC217414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501748, Dilution 1:50)
HEK293T cells transfected with either RC217414 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGAP25 antibody(TA501748), and then analyzed by flow cytometry.


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