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Home Antibody All anti-PEPD antibodies

Anti-PEPD TRUEMAB Antibody Clone OTI5D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501655 PEPD mouse monoclonal antibody, clone OTI5D5 (formerly 5D5) 100ul $379 In Stock
LC424818 PEPD HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501655 Carrier-free (BSA/glycerol-free) PEPD mouse monoclonal antibody, clone OTI5D5 (formerly 5D5) 100ug $450 3-4 weeks
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WB(2)
IHC(8)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell.
Clone NameClone OTI5D5 IsotypeIgG1
Species ReactivityHuman Concentration0.81 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:200 - 1:1000, FLOW 1:100
Predicted MW Explanation 54.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens peptidase D (PEPD), transcript variant 1
Alternative NamePROLIDASE
Database LinkNP_000276
Entrez Gene 5184 Human
FunctionThis gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.
Related PathwayProteaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (RC201970, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PEPD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PEPD mouse monoclonal antibody. (TA501655)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (TA501655)
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Flow cytometric Analysis of Hela cells, using anti-PEPD antibody(TA501655),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody(TA501655),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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