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Home Antibody All anti-PKLR antibodies

Anti-PKLR TRUEMAB Antibody clone 1F3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501412
  • anti-PKLR mouse monoclonal antibody, clone 1F3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400113) , 20ug
100ul 325 In Stock
CF501412
  • Carrier-free (BSA/glycerol-free) PKLR mouse monoclonal antibody, clone 1F3
100ug $450 3-4 weeks
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WB(1)
IF(1)
FC(1)
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Also for PKLR (NM_000298)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PKLR (NP_000289) produced in HEK293T cell.
Clone Nameclone 1F3 IsotypeIgG2a
Species ReactivityHuman Concentration0.97 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 1
Alternative NamePK1; PKL; PKR; PKRL; RPK
Database LinkNP_000289
FunctionThe protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.
IF Image
Anti-PKLR mouse monoclonal antibody (TA501412) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455).
FC Image
HEK293T cells transfected with either RC206455 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PKLR antibody(TA501412), and then analyzed by flow cytometry.

 

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