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Anti-EIF2S1 TRUEMAB Antibody Clone OTI1H1
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for EIF2S1 (NM_004094)
|Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.|
|WB, IHC, FC
||WB 1:2000, IHC 1:150, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)|
|EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A|
Entrez Gene 1965 Human
|The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501402)
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody(TA501402),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).