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Home Antibody All anti-CYB5R3 antibodies

Anti-CYB5R3 TRUEMAB Antibody clone 2A10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501353
  • CYB5R3 mouse monoclonal antibody, clone 2A10
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400140) , 20ug
100ul 379 In Stock
CF501353
  • Carrier-free (BSA/glycerol-free) CYB5R3 mouse monoclonal antibody, clone 2A10
100ug $450 3-4 weeks
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WB(2)
IHC(10)
FC(2)
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Also for CYB5R3 (NM_000398)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.
Clone Nameclone 2A10 IsotypeIgG2b
Species ReactivityHuman , Dog , Monkey Concentration0.76mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:200~500, IHC 1:150, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens cytochrome b5 reductase 3 (CYB5R3), transcript variant 1
Alternative NameB5R; DIA1
Database LinkNP_000389
FunctionThis gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (RC201592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501353)
FC Image
HEK293T cells transfected with either RC201592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYB5R3 antibody(TA501353), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-CYB5R3 antibody(TA501353),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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