Anti-CD5 Antibody, clone 6F7

Also for CD5 (NM_014207)

OriGene Data

Immunogen	Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
Clone Name	clone 6F7	Isotype	IgG1
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, FC	Suggested Dilutions	WB 1:500~2000, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens CD5 molecule (CD5)
Alternative Name	LEU1; T1
Database Link	NP_055022
Function	CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation (1). CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling (2).
Related Pathway	Hematopoietic cell lineage

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.

FC Image

HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA501334), and then analyzed by flow cytometry.

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Anti-CD5 TrueMAB Antibody clone 6F7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data