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Home Antibody All anti-GORASP1 antibodies

Anti-GORASP1 TRUEMAB Antibody Clone OTI5G8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501309 anti-GORASP1 (GRASP65) mouse monoclonal antibody, clone OTI5G8 (formerly 5G8) 100ul $379 In Stock
LC403127 GORASP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501309 Carrier-free (BSA/glycerol-free) GORASP1 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8) 100ug $450 3-4 weeks
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WB(2)
IHC(4)
IF(1)
FC(2)
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Also for GORASP1 (NM_031899)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.
Clone NameClone OTI5G8 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.6 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 46.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens golgi reassembly stacking protein 1, 65kDa (GORASP1), transcript variant 1
Alternative NameGOLPH5; GRASP65; P65
Database LinkNP_114105
Entrez Gene 64689 Human
Entrez Gene 56082 Rat
Entrez Gene 694728 Monkey
Entrez Gene 485594 Dog
FunctionThe Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GORASP1 (RC210076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GORASP1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501309, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501309, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501309, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GORASP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501309, Dilution 1:50)
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Anti-GORASP1 mouse monoclonal antibody (TA501309) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GORASP1(RC210076).
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HEK293T cells transfected with either RC210076 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GORASP1 antibody(TA501309), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-GORASP1 antibody(TA501309),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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