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Anti-FHL1 TRUEMAB Antibody Clone OTI2E11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501279 FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11) 100ul 325
In Stock
LC400563 FHL1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501279 Carrier-free (BSA/glycerol-free) FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell.
Clone NameClone OTI2E11 IsotypeIgG1
Species ReactivityHuman , Dog , Rat Concentration0.71 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 31.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens four and a half LIM domains 1 (FHL1), transcript variant 2
Database LinkNP_001440
Entrez Gene 2273 Human
Entrez Gene 25177 Rat
Entrez Gene 492162 Dog
FunctionThis gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.

IF Image
Anti-FHL1 mouse monoclonal antibody (TA501279) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).

FC Image
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501279), and then analyzed by flow cytometry.


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