Anti-IGF2BP2 Antibody, clone 3C8

Also for IGF2BP2 (NM_006548)

Gene Name	Homo sapiens insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), transcript variant 1
Synonyms:	IMP-2; IMP2; p62; VICKZ2
Immunogen	Full length human recombinant protein of human IGF2BP2(NP_006539) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 3C8	Isotype	IgG1
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Background	This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody. (TA501274, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody. (TA501274, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody. (TA501274, Dilution 1:50)

IF Image

Anti-IGF2BP2 mouse monoclonal antibody (TA501274) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2(RC205673).

FC Image

HEK293T cells transfected with either RC205673 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IGF2BP2 antibody(TA501274), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-IGF2BP2 antibody(TA501274),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-IGF2BP2 antibody(TA501274),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-IGF2BP2 TrueMAB Antibody clone 3C8

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