Anti-RANGAP1 Antibody, clone 1B4

Also for RANGAP1 (NM_002883)

Gene Name	Homo sapiens Ran GTPase activating protein 1 (RANGAP1)
Synonyms:	Fug1; SD
Immunogen	Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1B4	Isotype	IgG2a
Species Reactivity	Human , Dog , Rat	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Background	RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]
Related Pathway	Cell cycle

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (RC204223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (TA501265, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (TA501265, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (TA501265, Dilution 1:50)

IF Image

Anti-RANGAP1 mouse monoclonal antibody (TA501265) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1(RC204223).

FC Image

HEK293T cells transfected with either RC204223 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RANGAP1 antibody(TA501265), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-RANGAP1 TrueMAB Antibody clone 1B4

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