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Home Antibody All anti-RANGAP1 antibodies

Anti-RANGAP1 TRUEMAB Antibody clone 1B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501265
  • anti-RANGAP1 mouse monoclonal antibody, clone 1B4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401016) , 20ug Explanation
100ul
30ul
$379 In Stock
CF501265
  • Carrier-free (BSA/glycerol-free) RANGAP1 mouse monoclonal antibody, clone 1B4
100ug $450 3-4 weeks
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WB(3)
IHC(3)
IF(1)
FC(3)
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Also for RANGAP1 (NM_002883)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
Clone Nameclone 1B4 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat Concentration0.81 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 63.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Ran GTPase activating protein 1 (RANGAP1), transcript variant 2
Alternative NameFug1; RANGAP; SD
Database LinkNP_002874
Entrez Gene 5905 Human
Entrez Gene 362965 Rat
Entrez Gene 481234 Dog
FunctionRanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (RC204223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RANGAP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501265, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501265, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501265, Dilution 1:50)
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Anti-RANGAP1 mouse monoclonal antibody (TA501265) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1(RC204223).
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HEK293T cells transfected with either RC204223 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RANGAP1 antibody(TA501265), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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