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Home Antibody All anti-RANGAP1 antibodies

Anti-RANGAP1 TRUEMAB Antibody clone 1B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501265
  • anti-RANGAP1 mouse monoclonal antibody, clone 1B4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401016) , 20ug
100ul 379 In Stock
CF501265
  • Carrier-free (BSA/glycerol-free) RANGAP1 mouse monoclonal antibody, clone 1B4
100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(1)
FC(3)
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Also for RANGAP1 (NM_002883)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
Clone Nameclone 1B4 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat Concentration0.81mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Ran GTPase activating protein 1 (RANGAP1), transcript variant 2
Alternative NameFug1; RANGAP; SD
Database LinkNP_002874
FunctionRanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]
Related Pathway
Cell cycle

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (RC204223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501265, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501265, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501265, Dilution 1:50)
IF Image
Anti-RANGAP1 mouse monoclonal antibody (TA501265) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1(RC204223).
FC Image
HEK293T cells transfected with either RC204223 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RANGAP1 antibody(TA501265), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody(TA501265),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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