Anti-ACAT2 Antibody, clone 3C9

Also for ACAT2 (NM_005891)

Gene Name	Homo sapiens acetyl-CoA acetyltransferase 2 (ACAT2)
Synonyms:	OTTHUMP00000017527; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 2
Immunogen	Full length human recombinant protein of human ACAT2(NP_005882) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 3C9	Isotype	IgG2b
Species Reactivity	Human , Dog , Rat	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Background	The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2 .

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACAT2 mouse monoclonal antibody. (TA501223, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACAT2 mouse monoclonal antibody. (TA501223, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody. (TA501223, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (TA501223, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (TA501223, Dilution 1:50)

IF Image

Anti-ACAT2 mouse monoclonal antibody (TA501223) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 (RC201821 ).

IF Image

Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody (TA501223).

FC Image

HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(TA501223), and then analyzed by flow cytometry.

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Anti-ACAT2 TrueMAB Antibody clone 3C9

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