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Anti-ACAT2 TRUEMAB Antibody Clone OTI1G6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501220 anti-ACAT2 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6) 100ul 325
In Stock
LC417006 ACAT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501220 Carrier-free (BSA/glycerol-free) ACAT2 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6) 100ug $450 3-4 weeks
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Also for ACAT2 (NM_005891)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ACAT2(NP_005882) produced in HEK293T cell.
Clone NameClone OTI1G6 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.64 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 41.2 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens acetyl-CoA acetyltransferase 2 (ACAT2), transcript variant 1
Alternative NameOTTHUMP00000017527; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 2
Database LinkNP_005882
Entrez Gene 39 Human
Entrez Gene 708750 Monkey
FunctionThe product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Related PathwayDruggable Genome Fatty acid metabolismSynthesis and degradation of ketone bodiesValine, leucine and isoleucine degradationLysine degradationTryptophan metabolismPyruvate metabolismMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2 .

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.

IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501220, Dilution 1:50)

IF Image
Anti-ACAT2 mouse monoclonal antibody (TA501220) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 (RC201821 ).

IF Image
Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody (TA501220).

FC Image
HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(TA501220), and then analyzed by flow cytometry.


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