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Anti-ACAT2 TRUEMAB Antibody Clone OTI1C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501216 ACAT2 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) 100ul 325
In Stock
LC417006 ACAT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501216 Carrier-free (BSA/glycerol-free) ACAT2 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) 100ug $450 3-4 weeks
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Also for ACAT2 (NM_005891)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell.
Clone NameClone OTI1C5 IsotypeIgG2b
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.84 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 41.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens acetyl-CoA acetyltransferase 2 (ACAT2), transcript variant 1
Alternative NameOTTHUMP00000017527; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 2
Database LinkNP_005882
Entrez Gene 39 Human
Entrez Gene 308100 Rat
Entrez Gene 708750 Monkey
Entrez Gene 484063 Dog
FunctionThe product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq].
Related PathwayDruggable Genome Fatty acid metabolismSynthesis and degradation of ketone bodiesValine, leucine and isoleucine degradationLysine degradationTryptophan metabolismPyruvate metabolismMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
Anti-ACAT2 mouse monoclonal antibody (TA501216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2(RC201821).
Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody (TA501216).
HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(TA501216), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody(TA501216),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ACAT2 antibody(TA501216),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).


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